The effect of pre-existing maternal obesity on the placental proteome: Two-dimensional difference gel electrophoresis coupled with mass spectrometry

K Oliva; G Barker; C Riley; MJ Bailey; M Permezel; GE Rice; M Lappas

Journal article

The effect of pre-existing maternal obesity on the placental proteome: Two-dimensional difference gel electrophoresis coupled with mass spectrometry

K Oliva, G Barker, C Riley, MJ Bailey, M Permezel, GE Rice, M Lappas

Journal of Molecular Endocrinology | Published : 2012

DOI: 10.1530/JME-11-0123

Abstract

Our aim was to study the protein expression profiles of placenta obtained from lean and obese pregnant women with normal glucose tolerance at the time of term Caesarean section. We used two-dimensional difference gel electrophoresis (2D-DIGE), utilising narrow-range immobilised pH gradient strips that encompassed the broad pH range of 4-5 and 5-6, followed by MALDI-TOF mass spectrometry of selected protein spots. Western blot and quantitative RT-PCR (qRT-PCR) analyses were performed to validate representative findings from the 2D-DIGE analysis. Eight proteins were altered (six down-regulated and two up-regulated on obese placentas). Annexin A5 (ANXA5), ATP synthase subunit beta, mitochondria..

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University of Melbourne Researchers

Michael Permezel Author

Martha Lappas Author

Related Projects (2)

R. DOUGLAS WRIGHT BIOMEDICAL CAREER DEVELOPMENT AWARD - (UNDERSTANDING THE MECHANISMS THAT REGULATE THE ONSET OF HUMAN LABOUR AND DELIVERY)

Understanding the pathophysiology of Gestational Diabetes Mellitus

Gestational Diabetes Mellitus (GDM), which is defined as glucose intolerance of variable severity with onset or first recognition during pre..

Grants

Awarded by National Health and Medical Research Council (NHMRC)

Awarded by Australian NHMRC

Funding Acknowledgements

Dr M L was a recipient of a National Health and Medical Research Council (NHMRC) RD Wright Fellowship (grant number 454777). The work described in this manuscript was funded, in part, by a project grant from NHMRC (grant number 454310). Funding for the Chemi-Doc system, xMark Microplate Absorbance Spectrophotometer and Leica Qwin Image Analysis System was provided by the Medical Research Foundation for Women and Babies. Proteomic data analysis described in this work was supported by the use of the Australian Proteomics Computational Facility funded by the Australian NHMRC (grant number 381413).